Regulatory

Part:BBa_K549005:Design

Designed by: Sabine Wagner   Group: iGEM11_LMU-Munich   (2011-09-12)


LacI promotor fused with the iron dependent regulator fur


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 323


Design Notes

There where no special considerations we had to deal with.


Source

The lacI promotor is abstracted from the BB_J04500. The fur-regulator gene is amplified from Neisseria meningitidis.

References